Colleaques- A recent paper by M. Mack et al. (Nature Medicine 6:769, July 2000) documents transfer of CCR5 from positive to negative cells by membrane-derived microparticles. Other surface proteins, particularly 7-transmembrane spanners like chemokine receptors, may share this property. Transfer is relatively efficient and rapid (e.g. 1 hr.). We have observed similar effects in our experiments with cells transfected to express high levels of CCR5, which then quickly begins to appear on negative cells in the same culture. This is both a warning to be alert for this phenomenon in your experiments and a request for more information on other cell surface molecules which can translocate to negative cells. Don Mosier __________________________________ Donald E. Mosier, Ph.D., M.D. Department of Immunology-IMM7 The Scripps Research Institute 10550 North Torrey Pines Road La Jolla, CA 92037, USA ph 858 784-9121 fax 858 784-9190 NOTE AREA CODE CHANGE!
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