Hi, I do not think that anybody makes a full diagnosis of lymphoma with subclassification on a blood sample alone. In our Department in cases where there is splenomegaly but no enlarged lymph nodes, a fine needle aspiration of the spleen is performed with flow cytometry and/or immunocytochemistry on cytospins, bone marrow biopsy with smears and immunophenotyping is also done. Kind regards Anna At 08:39 2000-08-02 -0700, you wrote: >True, but there are other CD5-positive lymphomas (mantle cell) that may >mimic splenic marginal zone lymphoma in the blood and are much more >aggressive diseases. That is why I think it is unwise to make a diagnosis >of CD5+ SLVL on a blood sample alone. Rare cases may correlate with >splenic marginal zone lymphoma, but most will not. > > > ____________________Reply Separator____________________ > Subject: Re: Re[2]: interesting case > Author: Anja Porwit <Anja.Porwit@ks.se> > Date: 08/02/2000 1:13 AM > > > > Daniel! > Please look up Blood 1994 Mar 15;83(6):1558-62 > immunophenotype of splenic lymphoma with villous lymphocytes > and its > relevance to the differential diagnosis with other B-cell > disorders. > Matutes E, Morilla R, Owusu-Ankomah K, Houlihan A, Catovsky D > and several other articles. Both from the literature and our > own experience > there are rare cases that are CD5+ and correlate with the > morphological > diagnosis of SLVL. > Kind regards > Anna > > At 12:14 2000-07-31 -0700, you wrote: > > > >I don't think that CD5+ SLVL is a specific pathologic > diagnosis, and is > >unlikely to correlate with a tissue diagnosis of splenic > marginal zone > >lymphoma. > > > > Dan Arber > > City of Hope > > > > ____________________Reply Separator____________________ > > Subject: Re: interesting case > > Author: Anja Porwit <Anja.Porwit@ks.se> > > Date: 07/31/2000 4:59 AM > > > > > > > > > > Hello, > > CD5 can be positive in some cases of splenic lymphoma > with > > villous > > lymphocytes - could that diagnosis fit the cytology of > the > > cells in BM? Was > > CD11c positive? Tartrate resistant acid phosphatase? > > Best wishes > > Anna > > > > At 16:24 2000-07-27 -0400, you wrote: > > > > > >We have a case of an 80 year old male, with bone > marrow biopsy > > >showing lymphoproliferative process and enlarged > spleen. The > > bone > > >marrow tumor cells are reported cyclin D1 negative. > The case > > was > > >submitted with a diagnosis of hairy cell. WBC is 2.2 > with > > anemia and > > >thrombocytopenia. The peripheral blood is minimally > involved > > (7% of > > >lymphs). The tumor cells are CD19+, CD20 moderate (not > dim), > > CD22 > > >moderate, Immunoglobulin moderate- kappa/IgA IgM +, > CD5 > > positive but > > >spectrum from dim to moderate, FMC7 + and negative for > CD23, > > CD10, > > >CD103, CD25, CD38, IgD, IgG, and IgE. This doesn't > look like > > CLL or > > >mantle cell. We will be following up on the marrow > biopsy and > > further > > >clinical history and will let you know anything that > pops up. > > Has > > >anyone had a case like this? What is your favorite > diagnosis > > for this? > > > > > > > > > Maryalice Stetler-Stevenson and Doug Kingma > > >Maryalice Stetler-Stevenson > > >Director Flow Cytometry Unit > > >Laboratory of Pathology, NCI, NIH > > > > > Anna Porwit > > Hematopathology Lab. > > Department of Pathology > > Karolinska Hospital, Stockholm > > Anja.Porwit@ks.se > > tel.:+46-851774518 > > fax.:+46-851775843 > > > > > > > Anna Porwit > Hematopathology Lab. > Department of Pathology > Karolinska Hospital, Stockholm > Anja.Porwit@ks.se > tel.:+46-851774518 > fax.:+46-851775843 > > > Anna Porwit Hematopathology Lab. Department of Pathology Karolinska Hospital, Stockholm Anja.Porwit@ks.se tel.:+46-851774518 fax.:+46-851775843
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