Dear all We have been conducting studies on apoptosis in septic patients, using isolated PBMCs and neutrophils. Using Annexin V FITC (PharMingen 65874X), we found that all monocytes stain in the FL1 channel, around 10^3. We have been trying to find additional information on Annexin V staining of peripheral monocytes, but data is rather limited. In opposition to that, the only paper (Martín-Malo A et al. J Am Soc Nephrol 11:936,2000) did not find this background, although using Annexin V from other manufacturer (Bender MedSystems, Austria). Does somebody can help us to interpret what is going on? Thanks a lot. Esper G. Kallás Laboratório de Imunologia DIPA - EPM - UNIFESP kallas.dmed@epm.br São Paulo, SP
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