Re: alamar blue

From: tylee (tylee@itis.com)
Date: Wed Jul 12 2000 - 22:39:52 EST


Dr. Lavie,

Alamar blue is the dye known as resazurin. The trade marked name alamarBlue
includes some additional stabilizers that are described in a US patent
issued ~5 years ago (I think the patent is assigned to Alamar Biosciences;
but I think they are not in the research reagent business any more.)  There
are distributors that still sell the reagent; but I am away from my office
and don't have the info here in front of me and I can't remember who it is.

I think there was a lot of earlier work with this dye as a redox indicator
(resazurin -> resorufin) used to detect the level of bacterial contamination
in milk... perhaps dating back to the 50s or even earlier???

If you are simply looking for a redox indicator as an indicator of viable
cell number and you can accept a colorimetric readout in a multiwell format,
you might consider one or the tetrazolium reagents (MTT or MTS). If you are
simply looking for a fluorescent indicator of viable cells, you might
consider calcein-AM.

Ty Lee
-----Original Message-----
From: P.Lavie <plavie@techunix.technion.ac.il>
To: cyto-inbox
Date: Thursday, July 06, 2000 1:24 PM
Subject: alamar blue


>
>We are looking for nformation about alamar blue. Do you have any
>information where can we purchase it.
>Sincerely
>P Lavie
>Faculty of Medicine
>Technion Israel Institute f Technology
>



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