At 10:46 12/06/00 +0000, you wrote: > >Dear Flow group, > >I am currently having problems setting up a FACS Vantage for Hoechst Cell >cycle analysis. It has an Innova 70 laser with UV maximum at 50mW. I >would like to know what beads or set up methods others have used to >optimise the settings to get tight CV's. I have tried using UV beads from >Molecular Probes and then Calf thymus nuclei from the BD DNA QC particles >box with 5 ug/ml Hoechst, however, I cannot get any decent looking cell >cycle phases - I can only presume there are better methods than this >attempt. If anyone has any suggestions, it would be much appreciated. > >Regards >Natasha. > >Natasha Webb > >Assistant Flow Cytometry Operator >Room 5027 >Clinical Research Building >Hammersmith Hospital >London > >Phone: 0181 383 8330 > > > > Hello Natasha, Try fixed peripheral blood lymphocytes; Fix with 70% methanol @-20'C for one hour. Aspirate off the methanol with a vacuum line. stain. These should give a nice tight G0 peak. Alternatively, give me a call on the number below best regards Arnold _/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/ Arnold Richard Pizzey Department of Haematology Royal Free and University College London Medical School 98 Chenies Mews London WC1E 6HX U.K voice: +44 020-7679-6234 Fax: +44 020-7679-6222 email: a.pizzey@ucl.ac.uk _/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/
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