Dear Flow group, I am currently having problems setting up a FACS Vantage for Hoechst Cell cycle analysis. It has an Innova 70 laser with UV maximum at 50mW. I would like to know what beads or set up methods others have used to optimise the settings to get tight CV's. I have tried using UV beads from Molecular Probes and then Calf thymus nuclei from the BD DNA QC particles box with 5 ug/ml Hoechst, however, I cannot get any decent looking cell cycle phases - I can only presume there are better methods than this attempt. If anyone has any suggestions, it would be much appreciated. Regards Natasha. Natasha Webb Assistant Flow Cytometry Operator Room 5027 Clinical Research Building Hammersmith Hospital London Phone: 0181 383 8330
This archive was generated by hypermail 2b29 : Sat Mar 10 2001 - 19:31:23 EST