One thing you probably should check out is the stability of your laser. A noisy laser could make for very messy CVs, and I believe some I-70 lasers do have a problem in certain conditions. Regards, Joseph. At 20:46 12/06/2000, Natasha Webb wrote: >Dear Flow group, >I am currently having problems setting up a FACS Vantage for Hoechst Cell >cycle analysis. It has an Innova 70 laser with UV maximum at 50mW. I >would like to know what beads or set up methods others have used to >optimise the settings to get tight CV's. I have tried using UV beads from >Molecular Probes and then Calf thymus nuclei from the BD DNA QC particles >box with 5 ug/ml Hoechst, however, I cannot get any decent looking cell >cycle phases - I can only presume there are better methods than this >attempt. If anyone has any suggestions, it would be much appreciated. >Regards >Natasha.
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