Dear Flowers, It was clear for me, after I read the answers to my question about mean fluorescence channel analysis that most people suggested to analyze the data using the original number "mean" from the Log scale (10^0 - 10^4). However, I had analyzed most of my data after conversion of original data to channel values. Then, I start to have a problem because I did not want to go back and reanalyze all data since we have over 2,000 files to be analyzed. Therefore I was looking for a formula (mathematical expression) to convert the data back to Log values. Actually I found it on the CellQuest BD manual on chapter 6 "histograms". They say that to convert channel values to Log and vice-versa we just need to apply the following expression: Log values = 10^(channel value/*scale factor) where scale factor is 256 for a 0-1024 scale and 64 for a 0-256 scale. However, when I applied this formula to a group of data, that I had both values (Log and channel values), I did not find this formula as the right one to convert the data one to another. Does anyone have any experience with this. Best regards, Olindo
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