At 13:08 31/05/2000 -0700, you wrote: > >Greetings everyone, > >This is more genetics than flow, but here it goes: > >from my understanding CD25 binds to IL-2R on T cells and CD122 binds >to IL-2R on NK cells. > >Are the molecules distinct genetically? > >Do both consist of the alpha, beta and gamma chains? > >Does one antibody interfere with the other? I mean, if I were to do >staining with 25 and 122, is it better to do them in distinct tubes? > >also if anyone has any references type "review"(read- il-2r for >dummies) would you please forward me them? > >regards, > >maciej\ > >__________________________________________________ >Do You Yahoo!? >Send instant messages & get email alerts with Yahoo! Messenger. >http://im.yahoo.com/ > >-- End -- > Dear Maciej, The IL-2 receptor is actually made up of three seperate polypeptide chains, each coded by a different gene on a different chromosome. Beta (CD122) and gamma (CD132) chains are members of the haemopoietin receptor family with a highly conserved extracellular domain. The intracellular part is responsible for signalling pathways. The alpha chain (CD25) is structurally distinct and doesn't seem to be involved in direct intracellular signalling. Whereas the expression of the alpha chain is tightly regulated by mitogenic and antigenic stimulation, mainly due to the presence of a NF-kB element in its promotor region, the beta and gamma chains are constitutively expressed on resting lymphocytes. Thus, the alpha chain is expressed on activated T cells, B cells and macrophages. The beta chain is mainly expressed on the CD8 subset and on NK cells and monocytes. Combination of these receptor chains results in either the high affinity receptor (alpha+beta(+gamma)) or the intermediate affinity receptors (beta+gamma). A good book on cytokines and their receptors: Guidebook to cytokines and their receptors, edited by Nicos A. Nicola from Sambrook and Tooze (ISBN 0198599471). Best wishes, Joeri ------------------------------------- Joeri Aerts Experimental Laboratory Medicine - Laboratory Medicine (Haematology) CDG 7e VD - Gasthuisberg Herestraat 49 B-3000 Leuven BELGIUM tel.: +32-16347012 fax.: +32-16347042 e-mail: joeri.aerts@uz.kuleuven.ac.be -------------------------------------
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