Hello all, I would like some input regarding an issue I should probably be able to answer, but ... I have an investigator who has been using a FACSort to analyse his FITC/ PE/Cychrome labelled samples and gets good resolution of the populations of interest (dual positives). He feels that we have been unable to reproduce the results on our Vantage SE. We have moved the z axis so that the nozzle is a good inch above the laser intercept and this gives a good approximation of the results he has seen on the analyser. I have no problems with other samples and the sorter is performing very well. Is there something I can do to increase the sensitivity without rearanging the entire laser set up? The laser involved is an Enterprise II, with the primary power (UV) set to 25mW. This gives approx 300mW of 488 line. Thanks in advance, Rob McGilp Center for Neurologic Diseases Harvard Institutes of Medicine. Boston MA --------------------------------------------- This message was sent using RICS Webmail https://webmail.bwh.harvard.edu
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