Re:Dead Cell Removal

From: Janet Konyer (jkonyer@uoguelph.ca)
Date: Thu May 25 2000 - 12:30:15 EST


depending how pure it needs to be, you could aslo spin on a 6% BSA/PBS.


On Thu, 25 May 2000, Arnold Pizzey wrote:

>
>
> >
> >What is the most efficient method for removing dead cells from a single cell
> >suspension prepared from mouse spleens?
> >
> >Thanks in advance.
> >
> >Delynn Moss
> >Division of Parasitic Diseases
> >Centers for Disease Control
> >
> >
> Greetings Delynn,
>
> You might have a look at Miltenyi's website, they have an Apoptotic cell
> isolation kit
> which will do the trick.
>
>
> http://195.227.58.203/produkte/humreag/apoptotic.htm
>
> Regards,
>
>
> Arnold
>
> _/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/
>	Arnold Richard Pizzey
>	Department of Haematology
>	Royal Free and University College London Medical School
>	98 Chenies Mews
>	London WC1E 6HX
>	U.K
>
>	voice:	+44 020-7679-6234
>	Fax:	+44 020-7679-6222
>	email:	a.pizzey@ucl.ac.uk
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