Dear Delynn,
The most efficient method is a mini gradient centrifugation. All you
have to do is to lay your cells over ficoll-hypaque (or similar solution)
with a density of 1.077 in a Fisher tube and run it in a Fischer centrifuge.
If you contact any histocompatibility lab near you they will certainly be
able to help. If not let me know and I will send you the complete protocol.
Regards,
Jorge Neumann
Lab Transplantation Immunology
Santa Casa Hospital
Porto Alegre, Brasil
-----Mensagem original-----
De: Moss, Delynn M. <dmm3@CDC.GOV>
Para: Cytometry Mailing List <cytometry@flowcyt.cyto.purdue.edu>
Data: Quarta-feira, 24 de Maio de 2000 18:19
Assunto: Dead Cell Removal
>
>What is the most efficient method for removing dead cells from a single
cell
>suspension prepared from mouse spleens?
>
>Thanks in advance.
>
>Delynn Moss
>Division of Parasitic Diseases
>Centers for Disease Control
This archive was generated by hypermail 2b29 : Sat Mar 10 2001 - 19:31:21 EST