MOre along this line to one up the Dot Plot...density plots are great for recognizing small pops and close population centers -----Original Message----- From: Hans Jürgen Hoffmann [mailto:GRP02S.hjh@aaa.dk] Sent: Wednesday, May 24, 2000 3:48 AM To: cyto-inbox Subject: Re: analysis question - or dot plots vs histograms Dear Becky I take it that your question is not about the identification of eosinophils in whole blood. It is whether the dot plot method is preferable to the histogram method for analysing expression of surface molecules expressed at low density. I feel that your dot plot method is acceptable, as it approximates an Overton Subtraction result - see documentation for FCS Express (www.denovosoftware.com) or the literature for Roy Overtons publication of the method. The histogram method is preferred by traditionalists that are used to distinguishing CD4s from CD8s, and is not at all suitable for analysis of receptors expressed at low density. I would be curious to hear what the real pros have to say to this, though.
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