Re: Dead Cell Removal

From: Gillis Otten (Gillis_Otten@cc.chiron.com)
Date: Thu May 25 2000 - 12:37:44 EST 

     I would recommend centrifugation on a Ficoll-Hypaque gradient.  You
     should use a product such as Lympholyte-M (Cedarlane/Accurate) that
     has the appropriate density for mouse lymphoid cells.  Alternatively,
     there are recipes for preparing your own mouse-specific Ficoll-Hypaque
     (and I can send one to you if you like).

     Gib Otten, Ph.D.
     Chiron Corp
     Emeryville, CA 94608


______________________________ Reply Separator _________________________________
Subject: Dead Cell Removal
Author:  "Moss; Delynn M." <dmm3@CDC.GOV> at SMTP
Date:    5/23/00 2:44 PM



What is the most efficient method for removing dead cells from a single cell
suspension prepared from mouse spleens?

Thanks in advance.

Delynn Moss
Division of Parasitic Diseases
Centers for Disease Control

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