We routinely lyse bone marrow and have for many years. I believe it is recommended in the " U.S.-Candian consensus recommendations on the immunophenotypic analysis of hematologic neoplasia by flow cytometry" in CCC, vol 30, 1997, but someone stole my copy. Ficolling is yesterday. Maryalice >Content-Type: text/plain; charset=us-ascii; x-mac-type="54455854"; > >Content-Transfer-Encoding: 7bit > > During an exchange of messages regarding her posting of the Results of the >Clinical Practices Survey, Victoria Smith and I realized that neither of us >know whether whole blood lysis techniques are routinely applied to bone marrow >samples intended for immunophenotyping. > In the attempts conducted here, the results obtained using whole blood >lysis have been puzzling when compared with those using Ficoll separated bone >marrow cells. So, I am wondering whether others have compared the two >preparation methods on BMC and if they think whole blood cell lysis is good to >use on these cells. Are there references relating to this topic? > Also, does anybody still use Ficoll separation of peripheral blood cells >for clinical immunophenotypic analysis? > >Kevin G. Waddick, Ph.D. >Parker Hughes Cancer Center >2657 Patton Road >St. Paul, MN 55113 > > > >vbsmith@unmc.edu wrote: > > > In the survey, I did not ask or specify an alternative to cell lysis, so I > > do not know if laboratories still do ficoll separation. I would assume > > yes. I also did not query specimen type, so I do not know if whole blood > > cell lysis is used on bone marrow. But these are good questions and I wish > > I had thought to ask. :) > > Victoria Boryca Smith > > UNMC > > OB-GYN Research > >Received: by garnet.tc.umn.edu (mbox waddi002) > (with Cubic Circle's cucipop (v1.22 1998/04/11) Thu May 11 19:03:29 2000) >X-From_: vbsmith@unmc.edu Thu May 11 17:07 CDT 2000 >Received: from mhub1.tc.umn.edu by garnet.tc.umn.edu with ESMTP for >MIME-Version: 1.0 > >Return-Path: <vbsmith@unmc.edu> >Received: from UNMCNOTES.UNMC.EDU by mhub1.tc.umn.edu with ESMTP for >waddi002@tc.umn.edu; >Thu, 11 May 2000 17:07:44 -0500 >From: vbsmith@unmc.edu >To: cyto-inbox >Subject: Re: Results of the Clinical Pratices Survey >Date: Thu, 11 May 2000 17:08:09 -0500 >Message-Id: <OFCBF9A791.BAD48EDB-ON862568DC.00799885@UNMC.EDU> >X-MIMETrack: Serialize by Router on UNMCNOTES/Servers/UNEBR(Release >5.0.2b |December >16, 1999) at > 05/11/2000 05:08:11 PM >MIME-Version: 1.0 >Content-type: text/plain; charset=us-ascii >X-Mozilla-Status2: 00000000 > > >In the survey, I did not ask or specify an alternative to cell lysis, so I >do not know if laboratories still do ficoll separation. I would assume >yes. I also did not query specimen type, so I do not know if whole blood >cell lysis is used on bone marrow. But these are good questions and I wish >I had thought to ask. :) >Victoria Boryca Smith >UNMC >OB-GYN Research Maryalice Stetler-Stevenson Director Flow Cytometry Unit Laboratory of Pathology, NCI, NIH
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