During an exchange of messages regarding her posting of the Results of the
Clinical Practices Survey, Victoria Smith and I realized that neither of us
know whether whole blood lysis techniques are routinely applied to bone marrow
samples intended for immunophenotyping.
In the attempts conducted here, the results obtained using whole blood
lysis have been puzzling when compared with those using Ficoll separated bone
marrow cells. So, I am wondering whether others have compared the two
preparation methods on BMC and if they think whole blood cell lysis is good to
use on these cells. Are there references relating to this topic?
Also, does anybody still use Ficoll separation of peripheral blood cells
for clinical immunophenotypic analysis?
Kevin G. Waddick, Ph.D.
Parker Hughes Cancer Center
2657 Patton Road
St. Paul, MN 55113
vbsmith@unmc.edu wrote:
> In the survey, I did not ask or specify an alternative to cell lysis, so I
> do not know if laboratories still do ficoll separation. I would assume
> yes. I also did not query specimen type, so I do not know if whole blood
> cell lysis is used on bone marrow. But these are good questions and I wish
> I had thought to ask. :)
> Victoria Boryca Smith
> UNMC
> OB-GYN Research
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