Flow Gurus... Just a quick question regarding cytoplasmic staining: Do commercial preps such as BD's Cytofix/Cytoperm and Caltag's Fix and Perm also permiabilize the nuclear membrane? I'm interested in staining for a protein typically located within the nucleus which relocates to the cytoplasm when its cell is infected with an intra-cellular pathogen, so I need the nuclear membrane to remain intact to prevent false-positive signals. Any suggestions appreciated. Matt Matthew Morrow, MS, MT (ASCP) Warren Magnuson Clinical Center National Institutes of Health Bethesda, MD mmorrow@nih.gov/mmorrow@mail.cc.nih.gov (301) 496-4879
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