Re: cell line help

From: Rafael Nunez (rafaeln@vetvir.unizh.ch)
Date: Mon May 15 2000 - 03:01:32 EST


Maryalice, I use as last resourse to recover cell lines, to pass them into
peritoneal cavity of nude mice for three days and them wash the cavity with
medium and put back in culture. I also add a mixture of pen-strep (or
gentamicin) and fungizone. You can buy them as a full mixture form life
technologies or gifco. I hope this will help. Regards Rafael

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    ___/\_    | Rafael Nunez                  mailto:rafaeln@vetvir.unizh.ch
   /    o \/| | University Inst.for Virology  http://www.vetvir.unizh.ch/
  /        _| | Winterthurerstr. 266a         Telephone: (+41) 1 6358710
 /_/\__/-\/   | 8057 Zurich SWITZERLAND       Faximile : (+41) 1 6358911
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