Maryalice, I use as last resourse to recover cell lines, to pass them into peritoneal cavity of nude mice for three days and them wash the cavity with medium and put back in culture. I also add a mixture of pen-strep (or gentamicin) and fungizone. You can buy them as a full mixture form life technologies or gifco. I hope this will help. Regards Rafael \|/ (o o) ________________________________oOo__(_)__oOo_________________________________ ___/\_ | Rafael Nunez mailto:rafaeln@vetvir.unizh.ch / o \/| | University Inst.for Virology http://www.vetvir.unizh.ch/ / _| | Winterthurerstr. 266a Telephone: (+41) 1 6358710 /_/\__/-\/ | 8057 Zurich SWITZERLAND Faximile : (+41) 1 6358911 ______________________________________________________________________________
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