We have a cell line that a lot of work went into producing a stabile clone expressing a specific gene. Now, after a freezer blowout, the only cells left appear to be contaminated with some bacteria or small yeast. I know this is asking the impossible but has anyone got suggestions on how to clear this? The media has pen and step already. I am trying washing the cells repeatedly and bringing up in new media. Any other bright ideas? Please let me know ASAP- I'm on my last vial. Maryalice Maryalice Stetler-Stevenson Director Flow Cytometry Unit Laboratory of Pathology, NCI, NIH
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