Hi everyone. I want to study apoptosis in PBMCs using the staining with annexin as a marker of apoptotic cells. I have some questions regarding this issue: 1.- How good the annexin staining correlates with apoptosis. Can a cell be defined as apoptoic simply based in the annexin criterion? 2.- Is there any test rally specific for the fenomenon of apoptosis? 3.- What is the rationale of using propidium iodide in combination with annexin? Is it to differentiate apoptotic from necrotic cells? If this is the case, does it mean that cells positive for annexin and negative for PI should be classified as apoptotic and double positive cells as necrotic? Could you find cells positive for PI and negative for annexin? Sorry for so many questions and Thanks José Miguel benito Infectious Diseases Division Hospital Carlos III Madrid ________________________________________________________________________ Get Your Private, Free E-mail from MSN Hotmail at http://www.hotmail.com
This archive was generated by hypermail 2b29 : Sat Mar 10 2001 - 19:31:19 EST