Thanks to all the respondents(24)!! Here are the results. Victoria Boryca Smith UNMC OB-GYN Research vbsmith@unmc.edu Clinical Practices Survey Results 1. If a specimen is rejected due to age post draw, what is the cut-off time for rejection? A)>12 hours 1 4% B)>18 hours 0 0% C)>24 hours 8 33% D)>36 hours 2 8% E)>48 hours 9 38% No rejection 4 17% 2. On average, how old (post draw) is specimen when stained? A)0 Hours 4 17% B)1-6 hours 13 54% C)6-12 hours 1 4% D)12-24 hours 6 25% E)>24 hours 2 8% 3. Do you stain before or after lysis? BEFORE 17 71% AFTER 3 13% EITHER 3 13% 4. Which lysis method does your laboratory use? A)FACSlyse 13 54% B)ImmunoPrep 3 13% C)Immunolyse 2 8% D)Optilyse 0 0% E)Uti-lyse 0 0% F) NH4Cl 8 33% (1/2 use commercial preps) G) Other 2 8% 5. How many hours is specimen stored after staining before analysis? A)0 Hours 14 58% B)1-6 hours 7 29% C)6-12 hours 2 8% D)12-24 hours 2 8% E)>24 hours 3 13% 6. Is specimen fixed prior to analysis? Method? NO 6 25% FORM 4 17% PARAFORM 9 38% IMMUNOPREP(PARAFORM) 4 17%
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