Hi Rena We routinely use FL1-H versus FL2-H, both log. The only advantage I can see in using FL3 is that because of the wider separation of bandpass filters compensating out crossover would be less of a problem To your other query, we routinely use adherent cells in our annexin work. We find no problem in using trypsin/EDTA to detach cells, providing you return your cells to the incubator suspended in complete medium to allow the cells to recover from the detaching. It is important to handle the cells very gently: the assay depends absolutely on intact cell membranes. One point we have recently become aware of: don't vortex your cells too violently before running samples. We have seen some very odd results due to this! Eric P Miller Edinburgh Medical Oncology Unit "Everyone can be correct, all at the same time. That's the thing about quantum." Terry Pratchett, Lords and ladies On Wed, 3 May 2000, feinmarr wrote: > > Hi: > I need your input. > > What are the best parameters to use when acquiring samples for > FITC-annexinV and PI? In the past, our parameters were FL2-H vs FL1-H. > My colleague prefers using FL3-H vs FL1-H. What are the pros and cons > for both ways? We are using myeloma cell lines. > > My second question: can you perform annexin V binding on adherent cell > lines? > Thanks in advance, Rena > > > > > >
This archive was generated by hypermail 2b29 : Sat Mar 10 2001 - 19:31:18 EST