Re: CD62L

From: janet dow (jldow@unity.ncsu.edu)
Date: Fri May 05 2000 - 08:41:53 EST


Becton Dickinson makes a cd62L(l-selectin) ab  cat # 347447(called Leu-8)
that we use all the time on Cat lymphocytes in blood and lymph nodes.  it
works great but we have only had success using the PE conjugated form.  the
FITC conjugated ab does not work for us.

hope this helps



Janet Dow

At 12:41 PM -0400 5/3/00, Richard K. Meister wrote:
>Hello, all:
>
>I have a colleague who is interested in studying CD62L (L-selectin)
>expression on cat neutrophils using flow cytometry.  Do any of you know of
>a monoclonal, or polyclonal, antibody against CD62L that is specific for or
>cross-reacts with cat cells?
>
>Any leads would be greatly appreciated.
>
>Thanks in advance,
>
>Rick Meister
>* * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * *
>*  Richard K. Meister                  Email:  meister.1@osu.edu  *
>*  The Ohio State University           Voice:  (614) 292-9716     *
>*  Dept. of Veterinary Biosciences     FAX:    (614) 292-6473     *
>*  Cytometry Instrumentation Lab                                  *
>*  1925 Coffey Road                                               *
>*  Columbus, OH  43210  U.S.A.                                    *
>* * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * *


Janet Dow
Research Technician and Manager
Flow Cytometry Facility
North Carolina State College of Veterinary Medicine
Room C-314
Raleigh, NC 27606
(919)513-6364



This archive was generated by hypermail 2b29 : Sat Mar 10 2001 - 19:31:18 EST