I have a user who wishes to run Clontech EGFP and ECFP together; we can run EGFP O/K but we have not yet tried ECFP & I'm dubious.... Has anyone used ECFP (cyan) in a flow cytometer? If so, what laser & wavelength excitation? The Clontech website says excitation is 433 with a secondary absorption peak at 453. No spectra shown, so I don't know how broad the absorption curve might be. We don't have any 433 line available, would the argon 457 line work? Any ideas &/or experience? Thanks, Joseph. -- Joseph Webster, Flow Cytometry Facility Centenary Institute, Sydney AUSTRALIA.
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