You could not appreciate cell viability after fixation. It is unclear if you lyse red blodd cells or not. If not, you must do it. Try formol (1% in PBS), it works well for a 3 to 4 days delay. For more, try Stabilcyte from BioE. MSchwa3722@AOL.COM wrote : > Does anyone have a simple protocol for staining and fixing whole cord blood > for later acquisition. The samples I have run have shown significantly > decreased viability after fixation and delayed acquisition. We determine the > percentage of CD3 and CD34 positive cells based on the entire WBC population. > I suspect the cord blood granulocytes in the sample are contributing to the > decreased viability but due to the purpose of fixation for later acquisition > I would prefer not to have to ficoll the samples prior to staining. Perhaps > this is my only alternative. > > If anyone has any suggestions that may help I'd appreciate hearing from you.
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