I'm wondering why you are using FL3 instead of FL2 for propidium iodine? Would that effect the brightness? >>> Maciej Simm <simmmmer@yahoo.com> 04/18/00 05:14PM >>> Dear all, One of the people I'm working with right now is studying apoptosis in periteneal macrophages using a kit with annexin V monoclonal FITC ab and propidium iodine. The cells are autofluorescent. The "unstained" tube has signal in up to 2nd log. The stained tubes are VERY HIGHLY BRIGH on FL3 (PI) and I tried to make things "fit" on the dotplot by lowering fl3 voltage by 200 or so, but I'm still not getting everything (and also some of the dimmer cells are squooshed on the axis). The FITC signal is of normal brighness. Needless to say we're not getting the same results as the kit suggests we should given the incubation/conditions. He's gonna talk to the company. I am posting this message with hopes that some of you may have seen this method or one similar to it and are willing to comment. Any suggestions are welcome thanks a bunch, Maciej ===== `---------------------------------------------` | Maciej S. Simm | 525 E 68th Street | | Research Technician | Room N-805 | | Cornell Medical Center | Tel. 212.746.3428 | `---------------------------------------------` | www.cd4cd8.com | `---------------------------------------------` __________________________________________________ Do You Yahoo!? Send online invitations with Yahoo! Invites. http://invites.yahoo.com
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