Does anyone have a simple protocol for staining and fixing whole cord blood for later acquisition. The samples I have run have shown significantly decreased viability after fixation and delayed acquisition. We determine the percentage of CD3 and CD34 positive cells based on the entire WBC population. I suspect the cord blood granulocytes in the sample are contributing to the decreased viability but due to the purpose of fixation for later acquisition I would prefer not to have to ficoll the samples prior to staining. Perhaps this is my only alternative. If anyone has any suggestions that may help I'd appreciate hearing from you.
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