Re: Sorting of megakaryocytes?

From: B.Schmitz@biofrontera.de
Date: Tue Apr 18 2000 - 01:56:50 EST

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Hallo Dirk,

we established some years ago an easy, reliable and efficient
method for megakaryocyte isolation from human peripheral blood
and bone marrow (percoll gradient centrifugation and subsequent
MACS enrichment and/or FACS). You may contact Dr. Claudia
Wickenhauser for specific applications or the current protocols at
the Institute of Pathology, University of Cologne, Joseph-Stelzmann-
Str. 9, Germany (phone: 49 (0)221-478-5255/6368) or e-mail:
C.Wickenhauser@uni-Koeln.de.
Our procedures are also described in Schmitz et al. Eur J Haematol
1994, 53: 267-275. Moreover, Miltenyi Biotec offers additionally
CD61 MicroBeads for megakaryocyte isolation.
Good luck,
Beate

Next message: Hodge, Greg (HAEM): "RE: annexin V dim cells"
Previous message: Howard Shapiro: "Re: murine megakaryocytes staining"
In reply to: Dr. Dirk Behringer: "Sorting of megakaryocytes?"
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