> ---------- > From: Hodge, Greg (HAEM) > Sent: Monday, 17 April 2000 8:05 > To: Cytometry Mailing List > Subject: RE: annexin V dim cells > > > > > > ---------- > > From: shawn_jackson > > Sent: Wednesday, 12 April 2000 23:49 > > To: Cytometry Mailing List > > Subject: annexin V dim cells > > > > > > hey flow-ers > > > > I have a question concerning the occurrence of annexin V-dim cells vs. > > bright > > cells. I need help with references and/or data experience! > > > > I am staining peripheral blood from mice infected with vaccinia, and > then > > staining the CD8 cells with annexin V-fitc. I see a population of > annexin+ > > cells extending even up past the 2nd log at many time points, but at > > certain > > times I see very bright cells up at the 3rd log. > > > > I have been warned that only these very bright cells are truly AV+, > cells > > with > > any intentions whatsoever of doing anything related to a death process, > > while > > the dimmer cells represent actively proliferating cells only, with no > > intentions of dying. and these dimmer cells are likely to return to an > AV- > > population quickly. > > > > Could anyone help me with references or personal experince addressing > the > > status of these AV dim cells in contrast to bright ones? > > > > thanks! > > > > shawn jackson > > grad student > > harvard univ > > boston, ma > > > > Hello Shawn, > > I have recently described early apoptotic neutrophils from peripheral > > blood as Ann V dim and late apoptotic neutrophils as Ann V bright > (Optimal > > storage conditions for preserving granulocyte viability as monitored by > > Annexin V binding in whole blood. J Immunol Methods 225 (1999) 27-38. > > G.Hodge et al). You could try similar techniques with 7-AAD to determine > > if the CD8 Annex V dim cells are early apoptotic cells. > > Regards Greg Hodge. (hodgeg@mail.wch.sa.go.au). > > >
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