RE: annexin V dim cells

From: Hodge, Greg (HAEM) (hodgeg@mail.wch.sa.gov.au)
Date: Mon Apr 17 2000 - 18:41:27 EST


> ----------
> From:		Hodge, Greg (HAEM)
> Sent:		Monday, 17 April 2000 8:05
> To:	Cytometry Mailing List
> Subject:	RE: annexin V dim cells
>
>
>
>
> > ----------
> > From:		shawn_jackson
> > Sent:		Wednesday, 12 April 2000 23:49
> > To: Cytometry Mailing List
> > Subject:	annexin V dim cells
> >
> >
> > hey flow-ers
> >
> > I have a question concerning the occurrence of annexin V-dim cells vs.
> > bright
> > cells. I need help with references and/or data experience!
> >
> > I am staining peripheral blood from mice infected with vaccinia, and
> then
> > staining the CD8 cells with annexin V-fitc. I see a population of
> annexin+
> > cells extending even up past the 2nd log at many time points, but at
> > certain
> > times I see very bright cells up at the 3rd log.
> >
> > I have been warned that only these very bright cells are truly AV+,
> cells
> > with
> > any intentions whatsoever of doing anything related to a death process,
> > while
> > the dimmer cells represent actively proliferating cells only, with no
> > intentions of dying. and these dimmer cells are likely to return to an
> AV-
> > population quickly.
> >
> > Could anyone help me with references or personal experince addressing
> the
> > status of these AV dim cells in contrast to bright ones?
> >
> > thanks!
> >
> > shawn jackson
> > grad student
> > harvard univ
> > boston, ma
> >
> > Hello Shawn,
> > I have recently described early apoptotic neutrophils from peripheral
> > blood as Ann V dim and late apoptotic neutrophils as Ann V bright
> (Optimal
> > storage conditions for preserving granulocyte viability as monitored by
> > Annexin V binding in whole blood. J Immunol Methods 225 (1999) 27-38.
> > G.Hodge et al). You could try similar techniques with 7-AAD to determine
> > if the CD8 Annex V dim cells are early apoptotic cells.
> > Regards Greg Hodge. (hodgeg@mail.wch.sa.go.au).
> >
>



This archive was generated by hypermail 2b29 : Sat Mar 10 2001 - 19:31:16 EST