activated rat BAL macrophages, pt. 2

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Hi,

Somone suggested that to detect activated macrophages in rat bronchoalveolar
lavage we could use:

	1. anti rat mononuclear phagocyte antibody, 1C7 [with a cellular
distribution said to be similar to CD68 (ED1 antigen)]

plus, either,

	2. an anti Ia antibody such as RT1B,

or,

	3. anti rat OX-40L (described as a member of the TNF ligand superfamily)

Cost-wise and time-wise it would be best, of course, if we could use two
antibodies instead of three and I wondered if anyone agrees with this
conclusion, and, if so, would you pick RT1B or anti OX-40L to go along with
1C7?

Thanks in advance.

Ray Hester
Univ. of South Alabama

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