Olá Hai Qi, Thank you very much for your reply. I agree with you that B220 is not an absolute marker for B cells. However, what is so intriguing for me is why in my stimulated cultures, where I have an intense proliferation and a larger amount of "blast-large"cells, I do not see this phenomena. Then, if CD4+B220+ cells are activated T cells why in my Ag-stimulated cultures where I see proliferation (by thimidin incorporation assay) I do not see this unexpected phenotype. Many Thanks (muito obrigado), Olindo
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