-- Greetings everyone, We have to sort murine megakaryocytes. Can anyone recommend a good staining and is Hoescht 33342 staining a good solution to identify that population of less than 1%. In other words, can we really discriminate that 6N and more population ???? Would it be better to enrich the bone marrow by depleting other population by MACS. Thanks for your HELP Nathalie Tessier, M.Sc. Head of Flow cytometry Service Institut de recherches cliniques de Montréal 110 west, Pine Avenue Montreal, Quebec, Canada H2W 1R7 tel: 514-987-5608 fax:514-987-5736
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