Try this: De Luca C. The use of trypsin for the determination of cellular viability. Exp Cell Res. 1965 Oct;40(1):186-8. Margaret >Hi there!, > > >We as many others have been using trypane blue staining to account for >number og dead cells. > >We have tried in vain to find a basic reference that gives information as >to why the cells become trypane blue positive when they are dead. > >Can anyone help out?? > > >Thanks > > > >Peter Kierulf,MD PhD. Professor I > >Clinical Chemistry Dep. > >Ulleval University Hospital > >N-0407 OSLO, NORWAY > > >Telephone: +47 22 11 94 86 > >Telefax: +47 22 11 81 89 > >_-----------------------------------Mobil telphone: 922.85111 > >E-mail (RFC Formate): peter.kierulf@ioks.uio.no > >E-mai. (SA Formate): /G=peter/S=kierulf/OU=ioks/O=uio/PRMD=uninett/C=no > > >Could you please post this message, thanks > > > >Peter Kierulf,MD PhD. Professor I > >Clinical Chemistry Dep. > >Ulleval University Hospital > >N-0407 OSLO, NORWAY > > >Telephone: +47 22 11 94 86 > >Telefax: +47 22 11 81 89 > >_-----------------------------------Mobil telphone: 922.85111 > >E-mail (RFC Formate): peter.kierulf@ioks.uio.no > >E-mai. (SA Formate): /G=peter/S=kierulf/OU=ioks/O=uio/PRMD=uninett/C=no >>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>> Margaret M. Tropea Critical Care Medicine Department National Institutes of Health (ph) 301-496-7752 (fax) 301-480-3389 >>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>
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