Laura, We measure intracellular gIFN production from CD3/CD8 positive cells from nemestrina monkeys (pigtail). The gIFN antibody is from pharmingen (clone 4SB3) as is the anti CD3. The anti CD8 we obtain from BD. Pharmingen have a panel of antibodies that work with non-human primate cells, though i dont recall whether they have Abs for the other cytokines you mention. Nadeem Nadeem Sheikh, **************************************************************************************** RPRC Department of Pharmaceutics University of Washington University of Washington Box 357331 Box 357610 Seattle, WA 98195 Seattle, WA 98195 Correspondence 3000 Western Avenue Seattle, WA 98121 Voice : +1 206 221 2356 Fax : +1 206 956 0573 e-mail : nadeem@bart.rprc.washington.edu http://www.rprc.washington.edu/ ----- Original Message ----- From: Laura Vitale To: Cytometry Mailing List Sent: Tuesday, March 28, 2000 8:26 AM Subject: intracellular cytokine staining in non-human primates Hi! Can anyone give me information related to intracellular cytokine detection by flow cytometry in rhesus/macaque T cells? I'm specifically looking for information regarding human antibodies that cross-react (IL-2, TNF-a, IL-4 and g-IFN) and possible protocols for staining. Thanks, Laura Vitale Medarex, Inc Annandale, NJ 908-713-6000 x338
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