Dear Dr. Zucker, >Is it possible to measure viability in a cell population and then fix the >cells prior to analysis by flow cytometry. [...] > Does any one have any knowledge or information on this type of protocol. Levelt CN, et al. Streptavidin-tricolor is a reliable marker for nonviable cells subjected to permeabilization or fixation. Cytometry. 1994 Jan 1;15(1):84-6. I hope this information is helpful Best regards Dr. L. VOLKOV Ass. en Cytometrie -Microscopie Serv. d'Immunologie Fac. de medecine 3001, 12 av.Nord SHERBROOKE (Quebec); CANADA J1H 5N4 Tel: (819) 346-1110 ext 4867 Fax: (819) 564-5215 Leonid Volkov <leonidv@crccuse.usherb.ca>
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