Yes--it is possible! I had excellent results using 7-AAD to stain dead cells prior to fixation. The leakage of 7-AAD to live cells can be blocked by using non-fluorescent actinomycin D in the media. I followed the technique outlined by Schmid et al. (1999) in Cytometry 35:64-74. Julie Oughton Oregon State University > > > >At a recent flow cytometry workshop in the Research Triangle Park area the >following question was asked: Is it possible to measure viability in a cell >population and then fix the cells prior to analysis by flow cytometry. Can the >dye stay in the dead cells without leaking into the live cells? I remember that >Dr. Allan Pollock worked on these techniques in the 1980's . Does any one have >any knowledge or information on this type of protocol. The research groupe wants >to apply this technique on AIDS blood. Thanks >Bob > >Robert M. Zucker, Ph.D >U.S. Environmental Protection Agency >National Health and Environmental Effects Research Laboratory >2525 NC Highway 54, MD 72 >Research Triangle Park, North Carolina, 27709 >tel: 919-541-1585 fax 919-541-4017 >e-mail: zucker.robert@epa.gov > > >
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