Perhaps you should decrease the concentration of Rhodamine or decrease the incubation time. We follow the orpogen protocol for BurstTest. Also I recommend Sodium Hep instead of Lithium. Lithium forms larger ions ... which probably means something if you're a cell. --- Helena Marečková <helena.mareckova@lf1.cuni.cz> wrote: > > We have recently started to look at oxidative burst as a means of > assesing phagocytosis. We use whole-blood method with DHR123 as a > substrate which is converting to rhodamine during oxidative burst. > Our problem is in high spontaneous metabolic activity of PMN's: in > the > polypropylene tube with lithium-heparin whole blood + DHR123 +NaCl > 0,9% > we have after incubation and lysis too high intensity of > fluorescence > FL1. Does anyone have experiences with this problem? > Thanks for your help. > Karin Malickova > kmali@lf1.cuni.cz > > ===== `---------------------------------------------` | Maciej S. Simm | 525 E 68th Street | | Research Technician | Room N-805 | | Cornell Medical Center | Tel. 212.746.3428 | `---------------------------------------------` | www.cd4cd8.com | `---------------------------------------------` __________________________________________________ Do You Yahoo!? Talk to your friends online with Yahoo! Messenger. http://im.yahoo.com
This archive was generated by hypermail 2b29 : Sat Mar 10 2001 - 19:31:12 EST