HI Helena, Have you looked into a kit made by Orpegen? They make two kits called "Phagoburst" and "Burst Test". They have controls and instructions for flow setup. The contact is Ahos Garhofman, email "Ahosmann@orpegan.com". Donna Helena Marečková wrote: > We have recently started to look at oxidative burst as a means of > assesing phagocytosis. We use whole-blood method with DHR123 as a > substrate which is converting to rhodamine during oxidative burst. > Our problem is in high spontaneous metabolic activity of PMN's: in the > polypropylene tube with lithium-heparin whole blood + DHR123 +NaCl 0,9% > we have after incubation and lysis too high intensity of fluorescence > FL1. Does anyone have experiences with this problem? > Thanks for your help. > Karin Malickova > kmali@lf1.cuni.cz
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