sorting of retrovirus transduced cells

From: Robert Pawliuk (rpawliuk@genetixpharm.com)
Date: Tue Mar 14 2000 - 14:39:32 EST


Help.

I'm looking for a flow cytometry facility within the greater Boston area
which would be able to sort murine bone marrow cells transduced with
recombinant lentiviral vectors carrying a gene encoding the green
fluorescence protein. These vectors DO NOT harbor any Lentiviral genes and
DO NOT produce any Lentiviral proteins. All  transduced bone marrow cells
are tested for the presence of any replication competent Lentivirus and
NONE has ever been detected (< 20 experiments). Any information that
anybody has would be greatly appreciated.  Thanks in advance.

Rob Pawliuk



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