NK cells are known to have fairly high levels of Pgp (multidrug resistance protein) which is known to pump out substrates like INDO. Try loading for 30 min and then holding on ice until actual use on the cytometer. Phil Marder Lilly Research >From: Sonja Rotzoll <sonja.rotzoll@mail.uni-wuerzburg.de> >To: Cytometry Mailing List <cytometry@flowcyt.cyto.purdue.edu> >Subject: NK cells and Ca-flux >Date: Thu, 9 Mar 2000 16:51:38 +0100 (MET) > > > >Dear flowers! > >We have problems to measure Ca-flux of fresh mouse NK cells. The cells are >loaded with Indo. The loading of the NK cells is not so fine like the T >cells. > >The T cells show nice peaks if they are stimulated with Ionomycin. The NK >cells of the same sample show just a little reaction with Ionomycin. > >Do you have an idea why the NK cells don't show the same reaction? >Do you know how to get a better loading of the NK cells? Or should we try >another dye? > >Thanks for your hlep! > >Sonja > >******************************************************************************* >Sonja Rotzoll >Institut fuer Virologie und Immunbiologie >Versbacher Str.7 >97078 Wuerzburg, Germany > >e-mail: sonja.rotzoll@mail.uni-wuerzburg.de > ______________________________________________________ Get Your Private, Free Email at http://www.hotmail.com
This archive was generated by hypermail 2b29 : Sat Mar 10 2001 - 19:31:11 EST