How fast do your Elite ESP's Sort? 10x faster than not very fast isn't very fast. So I'm curious. thanks, chris On Fri, 25 Feb 2000 08:49:36 -0500, Mark A. KuKuruga wrote: > >Andreas, >Our BD FACS Vantage SE has been in operation for several months. I ve >heard from others that initial startup following installation has been a >problem, but that s not been the case here . . . we were fully >operational in about a month. BD, as usual, has been very responsive to >questions and concerns, and this has helped considerably. >At this point, I find the Vantage to be very stable, with sensitivity >comparable to other flow cytometers I ve worked with. Purity and >recovery is always very good. Considering sort rate and recovery, our >Vantage sorts about 10x faster than our Elite ESPs. Sort setup >typically takes about 15 minutes (I can do it in 5, when I m >desperate). So far, I have very little to complain about. >BD s OmniComp (inter-beam compensation) works well, but I think it s >fair to say that this component is still being developed. It does >compensate 633 excitation of the CY part of PE-CY tandems very >effectively, but not all compensation combinations are possible. You >sometimes have to be creative with detector configurations. BD s >expanded Detector Options allow this, and I would recommend including >these options to anyone considering a Vantage. >I use BD s CloneCyt their plate sorting system -- frequently (almost >daily) for sort setup and actual plate sorting. Since a recent software >upgrade, it has performed flawlessly. >The CellQuest software works well and pretty much does everything we >need it to do. Again, BD has responded well to the minimal concerns >I ve had. I m still contemplating data archival. I don t trust Zips, >so eventually I ll install a CD-Recorder. BD is supposed to be working >on a recommendation . . . >I would say that generally, the Vantage has performed well, and >certainly does all that BD claims. I would recommend it as a definite >advantage to any flow sorting operation. >Have fun. >MAK. >-- >Mark A. KuKuruga, Managing Director >University of Michigan Core Flow Cytometry ><http://www.cancer.med.umich.edu/flow_cytometry> >phone: 734-647-3216 fax: 734-936-7376 >kukuru@umich.edu > >Andreas Simm wrote: > >> Hello everybody, >> I know that this question appears nearly all three month, but I now >> have to decide which high speed sorter we >> should buy. As we will have only one apparatus for both, analysis and >> sorting, the decision (may be) will not be the >> same as if one consider to buy a flow cytometer for sorting purposes >> only. >> >> And as far as I know, almost only MoFlo user answered to the last >> queries. >> So my questions: >> What is the experience with the Altra (Coulter)? I did not hear from >> any experience in this list. >> What is the experience with the FACS-Vantage? >> From the last queries, the MoFlo tends to be the best sorter (but only >> users of the MoFlo answered!). What's the >> experience with it for analysis purposes only. Is it easy to handle? >> Is anybody in the flow community, who can really compare these >> sorters? >> >> Thanks for every statement in advance! >> >> Andreas >> >> My address: >> >> PD Dr. Andreas Simm >> Institut fuer Klinische Biochemie und Pathobiochemie >> Versbacher Str. 5 >> D-97078 Wuerzburg >> Germany >> >> Tel.: +49 931-201-3144 >> FAX.: +49 931-201-3153 >> E-mail: simm@klin-biochem.uni-wuerzburg.de > >
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