Dear flowers! We have problems to measure Ca-flux of fresh mouse NK cells. The cells are loaded with Indo. The loading of the NK cells is not so fine like the T cells. The T cells show nice peaks if they are stimulated with Ionomycin. The NK cells of the same sample show just a little reaction with Ionomycin. Do you have an idea why the NK cells don't show the same reaction? Do you know how to get a better loading of the NK cells? Or should we try another dye? Thanks for your hlep! Sonja ******************************************************************************* Sonja Rotzoll Institut fuer Virologie und Immunbiologie Versbacher Str.7 97078 Wuerzburg, Germany e-mail: sonja.rotzoll@mail.uni-wuerzburg.de
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