Hi Kevin... Caltag now has APC-Cy7 -conjugated antibodies against human CD8 and CD45. We use their APC-Cy7-anti-mouse CD45R (B220) antibody and it works well. I believe they are expanding their selection of APC-Cy7 conjugates - its probably worth checking with them to see if CD19 is on their list. They may also be able to do a custom conjugation for you. Bill At 05:07 PM 3/9/00 -0600, Kevin Waddick wrote: > > We are very interested in using antibodies to CD7 or CD19 that are >directly conjugated to the APC-Cy7 fluorochrome. Unfortunately, I >haven't been able to find a company that sells either of these. On the >other hand, we have produced our own monoclonal antibodies to CD7 and >CD19 that we conjugate to FITC and PE. > I am wondering if anyone out there knows of a source of the APC-Cy7 >fluorochrome itself from which we can obtain it. We would then be able >to link it to our own antibodies. I am also interested in finding a >source for obtaining PE-Cy7. As always, any assistance is greatly >appreciated. > >Kevin G. Waddick, Ph.D. >Parker Hughes Institute >2657 Patton Road >St. Paul, MN 55113 >
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