Just out of curiousity: we do Ca++ mobilisation with an Enterprise laser set at 20mW UV, but in visible light regulation mode. It is a fairly new model / laser, with about 6 months use... and of course, well warmed up. However, it varies quite a bit in UV output over time: say, after setting at 20mW it can vary around 23mW +/- 5mW. (In UV regulation, the 488 output varies enough that populations may be shifted outside the gate; of course, 488 output is very stable in visible light regulation mode, as is UV output in UV regulation mode.) This is ok for indo ratio-ing, but probably not so hot for DNA and other more critical applications. I was hoping you might give me a range of what you have found for stability of UV output in visible light regulation mode... I'm wondering if our Enterprise wanders more than most, or if this is typical of the Enterprise. Thanks very much, Claude
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