In my intracellular staining protocol, PMA/ionomycin stimulation is usually done the day after blood collection and PBMC ficoll separation. Does anyone know what is the best overnight storage temperature for PBMC? I did 37 °C, 5 % CO2 and also closed tubes at 4°C, both in RPMI supplemented with BCS. For certain cytokines these 2 conditions really give different results! Thank you for helping, Best regards, Wim Jennes --------------------------------------------------- Wim Jennes Institute of Tropical Medicine Department of Microbiology Laboratory of Immunology Nationalestraat 155 B-2000 Antwerpen - Belgium Tel: + 32 (0)3 247 62 27 Fax: + 32 (0)3 247 62 31 e-mail: wjennes@itg.be ---------------------------------------------------
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