mouse intracellular cytokines

From: Margaret Tropea (mtropea@nih.gov)
Date: Fri Feb 25 2000 - 08:12:34 EST


I have a visiting medical fellow from Denmark that would like to do IFNg
and IL2 intracellular staining by flow on mouse splenocytes and lymph
nodes.  I've only run human samples (whole blood, isolated cells, cultured
cells but not lymphs) and not for these particular cytokines.  Any
information would be greatly appreciated.
Thanks!
Margaret
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Margaret M. Tropea
Critical Care Medicine Department
National Institutes of Health
(ph) 301-496-7752
(fax) 301-480-3389
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