Cathleen, the latter of these two references will give you a good start. I haven't read the first one yet, but it may also have some useful information for you. 1. Vaddi, K. and Newton, R.C. Regulation of monocyte integrin expression by beta-family chemokines. J.Immunol. 153(10):4721-4732, 1994. 2. Murphy, F.J. and Reen, D.J. Differential expression of function related antigens on newborn and adult monocyte subpopulations. Immunol. 89:587-591, 1996. Craig Turner International Blood Group Reference Laboratory Bristol UK -------- From: MayeskiC@allkids.org To: cytometry@flowcyt.cyto.purdue. Subject: Leukocyte Adhesion Molecules Date: Wednesday, February 16, 00 21:41 Please help, I am in search of any information regarding flow protocols to analyze leukocyte adhesion molecules. Our doctors order CD18, 11a, 11b, 11c and CD54 on our pediatric patients. I have been unable to find references or anyone with experience with this assay. We have been performing surface marker analysis and comparing to a normal patient control sample. Any information on gating, normal ranges, antibody combinations, reporting protocol, or a resource would be greatly appreciated. Thank you for your help, Cathleen Mayeski IPL @ All Children's Hospital Med Tech/Supervisor
This archive was generated by hypermail 2b29 : Sat Mar 10 2001 - 19:31:07 EST