Please help, I am in search of any information regarding flow protocols to analyze leukocyte adhesion molecules. Our doctors order CD18, 11a, 11b, 11c and CD54 on our pediatric patients. I have been unable to find references or anyone with experience with this assay. We have been performing surface marker analysis and comparing to a normal patient control sample. Any information on gating, normal ranges, antibody combinations, reporting protocol, or a resource would be greatly appreciated. Thank you for your help, Cathleen Mayeski IPL @ All Children's Hospital Med Tech/Supervisor
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