Dear flowers, Mike Evans's logic seems right if the machine is set up right a single drop envelope with one cell in it should work. Two points 1.In the Clone Cyt user guide it only says optimise the sorter for sorting. On page 24 it shows the sort control window with 3 deflected drops and counter Sort Mode. 2.In the Vantage user guide when in Counter mode the 3 drop envelope(shown in the picture) is extended to 4 "to achive high count accuracy" From my own experience a three drop envelope gives better purity over a wider range of Dop Delays than a 2 or 1 drop delay.With a one drop envelope drop delay must be spot on, any drift and purity is lost.I'm sure lots of people reading this with ageing Vantages get some varation in drop delay it seems to me this is being compensated for by a three drop envlope.What do other people use? A friend of mine with a new Vantage SE uses a 1.5 drop envelope I dont see the logic in this as half the time it sorts 2 drops and half the time it sorts 1!Which is best? Geoff Morgan Babraham, Cambridge UK
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