Julia, I think that you need to run cytoplasmic CD22 and/or CD79a as well as TdT. This can still be a B-precursor ALL with weak expression of CD19. Which conjugate did you use for CD19? if you used FITC you may want to repeat the staining with CD19PE. Best wishes Anna At 16:06 2000-01-24 GMT+100, you wrote: > >Dear Flow-ers, >We got a bone marrow sample from a 5-year-old boy who has anaemia, >thrombopenia and leukopenia, returning febrile status, leg pain. >We found that only 10% of bone marrow cells expressed CD45. 90% of >the blast-like cells were HLA-DR+ and CD10+, but they do not express >CD19. The percentage of CD34+ cells are also very low (0.1%). We >could not detect neither CD33 or CD13. 5% of the cells were positive >for CD3, CD5 or C7. >What kind of malignancy can this be? >I would appreciate any comments. >Julia > >Julia B. Szeberenyi M.D >Dept. of Genetics, Cell-and Immunobiology >Laboratory of Flow Cytometry >Semmelweis University of Medicine >H-1089 Budapest, Nagyvarad ter 4 >Hungary >email: szebjul@net.sote.hu >fax:+36-1-303-69-68 > Anna Porwit Haematopathology Lab. Department of Pathology Karolinska Hospital, Stockholm anpo@mb.ks.se tel.:+46-851774518 fax.:+46-851775843
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